《植物生理学报》 2017, 53(5): 785-794
通信作者:陈松笔;E-mail: songbichen@catas.cn
摘 要:
以木薯(Manihot esculenta)栽培种‘华南5号’ (SC5)为研究材料, 利用双层黑塑料袋造成暗光环境, 木薯在暗光环境分别生长0、1、3、9 d和暗光环境9 d后见光4 h, 采用蛋白质组学方法, 揭示与光合作用相关的蛋白质表达水平变化。结果表明: 随着暗光环境处理天数的增加, 叶绿素总含量整体呈下降趋势; 以0 d暗光环境处理的木薯叶片蛋白质图谱为对照, 通过其他暗光环境处理共得到45个平均差异表达量为对照±2.0倍以上的蛋白质, 它们的生物学功能涉及光合作用、碳和能量代谢、结构、解毒、抗氧化等。而ATP合酶、核酮糖-1,5-二磷酸羧化酶/加氧酶(RuBisCO)活化酶、RuBisCO大亚基、磷酸核酮糖激酶前体、光系统I亚基及RuBisCO小亚基前体随着暗光环境处理天数延长而下调, 显示这些蛋白质的表达水平与光合作用强弱密切相关。关键词:木薯; 叶片; 暗光环境; 叶绿素含量; 蛋白质组
收稿:2017-04-06 修定:2017-05-04
资助:国家自然科学基金委员会与国际农业磋商组织(NSFCCGIAR)国际合作重点项目(31361140366)、2012年海南省高层次创新创业人才启动基金(HLCT-HN2012-2)和“十二五”农村领域国家科技计划课题(2015BAD15B01)。
Corresponding author: CHEN Song-Bi; E-mail: songbichen@catas.cn
Abstract:
The expression changes of photosynthesis-related proteins were determined using proteomic method extracted from the leaves of cassava (Manihot esculenta) ‘South China No. 5’ (SC5), covering with double black plastic bags to create a dark environment, under 0, 1, 3 and 9 days dark environments and 9 day dark environments plus light condition for 4 hours. The results show that the total chlorophyll contents of SC5 cassava leaves decreased with the increase of treated days under dark environment. A total of 45 differential expression proteins were found to change significantly with larger than ±2.0 times variation intensity at 4 dark treatments (1, 3, 9 days and 9 days plus 4 hours light) compared to 0 day. These differential proteins were identified to involve in photosynthesis, carbohydrate and energy metabolism, structure, detoxification and antioxidation, etc. However, the expressions of ATP synthase, ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) activase protein, RuBisCo large subunit, phosphoribulokinase precursor, photosystem I subunit and RuBisCo small chain precursor down-regulated with the increase of treated days under dark environment.Key words: Manihot esculenta; leaf; dark environment; chlorophyll content; proteome
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